KMID : 0829320130160010033
|
|
Korean Journal of Clinical Microbiology 2013 Volume.16 No. 1 p.33 ~ p.38
|
|
Detection of 13 Enteric Bacteria and 5 Viruses Causing Acute Infectious Diarrhea Using Multiplex PCR from Direct Stool Specimens
|
|
Lee Seung-Ok
Park Yeon-Joon Yoo Jin-Kyung Lee Hae-Kyung Kim Ja-Young Lee So-Young Kim Soo-Young
|
|
Abstract
|
|
|
Background: We investigated the prevalence of various pathogens (13 enteric bacteria and 5 viruses) which cause diarrhea using multiplex PCR of stool specimens and compared two multiplex PCR methods for detecting diarrheagenic Escherichia coli.Methods: A total of 405 stool specimens submitted between November 2010 to February 2011 for routine culture of enteric pathogens were included and screened for five viruses (astrovirus, Group A rotavirus, enteric adenovirus, norovirus G1/G2) and eight bacteria (Salmonella spp., Shigella spp., Campylobacter spp., Vibrio spp., C. difficile Toxin B, C. perfringens, Y. enterolytica, Aeromonas spp.) using the Seeplex¨Þ Diarrhea ACE detection kit (Seegene). In addition, virulence-associated genes of enteropathogenic E. coli,(EPEC), enterohemorrhagic E. coli (EHEC), enteroinvasive E. coli, (EIEC), enterotoxigenic E. coli (ETEC), and enteroaggressive E. coli (EAEC) were detected using 16-plex PCR and a commercial diarrheagenic E. coli detection (DEC) PCR kit (SSI Diagnostica).Results: Overall, 138 (34.1%) of 405 samples was positive for pathogen. The positive rate for virus was18.5%. norovirus G2, Group A rotavirus, enteric adenovirus, astrovirus and norovirus G1 were detectedin 40, 23, 8, 3 and 1 samples, respectively. The positiverate for bacteria was 24.4% (99/405). C. difficile toxin B was the most frequently detected, followed by C. perfringens, EPEC, and EAEC. The agreements of the two multiplex PCR methods for detecting EPEC and EHEC were 99.3% and 100%, respectively.
Conclusion: The detection rate was high (34.1%) including various diarrheagenic E. coli (6.2%) and C.perfringens (5.2%). Multiplex PCR is thus useful for detecting various pathogens. (Ann Clin Microbiol 2013;16:33-38)
|
|
KEYWORD
|
|
Agreement, Diarrheagenic Escherichia coli, Norovirus, Rotavirus
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|